Thursday, July 06, 2006

Harlan

MY BABY MUNTANT E-COLI SURVIVED! I mean who would have thought it. yesterday (wednesday) we moved our little E-coli into other containers chalked full of LB Agar solution (some really smelly discusting gel like substance that the E-coli like to eat...eew), but at the same time it was also chalked full of anti-biotics. We made it so the GM (Genetically Modified) E-coli bacteria were imune to ampicillin by putting a specific gene in with th pGLO gene (the one that makes 'em glow under UV light)this gene was called the Bla gene. It made the e-coli release enzymes that cut up the ampicillin effectivly destoying it so the E-coli were able to live in such an environment. And today after lunch we made them GLOW! It was very fun.

4 comments:

Scotty Dog said...

Hurray... only 3 of mine and Byran's survived... but with bacteria, give it a day and many more will show up!

Damian said...

that was kind of funny. You did put 50 of whatever, right?

Scotty Dog said...

*shrugs* I had thought so...but whatever.

XLiquidIceX said...

Congrats, here in the West Coast, we are almost ready to make them glow. Today, we did the biotransformation with the E.coli and the pGLO DNA. I hope for good results tomorrow.